Nitrogen spiking is simply a way to manipulate the test used to measure the protein content of protein powders. The current method used to measure the amount of protein in a protein powder involves measuring the nitrogen content, which is then converted into protein amount. Nitrogen is used because protein is made up of amino acids that are strung together in a chain, much like a pearl necklace. Every amino acid contains nitrogen, so measuring nitrogen content of a protein powder can indicate the amount of protein it contains per serving. But that’s assuming that the protein powder contains just complete proteins, such as whey, casein and/or egg protein. Unfortunately, many protein powders not only include these complete proteins, but they also have “extra” amino acids added to them.buy now!